Severe acute respiratory syndrome coronavirus accessory proteins 6 and 9b interact in vivo.
Identifieur interne : 001C55 ( Main/Exploration ); précédent : 001C54; suivant : 001C56Severe acute respiratory syndrome coronavirus accessory proteins 6 and 9b interact in vivo.
Auteurs : Enrique Calvo [Espagne] ; Marta L. Dediego ; Pilar García ; Juan A. L Pez ; Pilar Pérez-Bre A ; Ana Falc NSource :
- Virus research [ 1872-7492 ] ; 2012.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Viral Regulatory and Accessory Proteins.
- physiology : SARS Virus.
- Amino Acid Sequence, Animals, Chlorocebus aethiops, Immunoprecipitation, Mass Spectrometry, Molecular Sequence Data, Protein Interaction Mapping, Vero Cells.
Abstract
The 3'proximal one-third of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes the structural proteins and eight accessory proteins, including 3a, 3b, 6, 7a, 7b, 8a, 8b and 9b, varying in length from 39 to 274aa which do not share significant homology with viral proteins of known coronaviruses. The SARS-CoV protein 6 is 63 amino acids in length and has been previously involved in virus pathogenicity and replication. To further analyze this functions, the interaction of SARS-CoV protein 6 with other viral and/or cellular factors has been analyzed during SARS-CoV infective cycle. Protein 6 immunoprecipitation from extracts of SARS-CoV infected cells and mass spectrometry analysis revealed an interaction of viral proteins 6 and 9b in biologically relevant conditions. This interaction has been reinforced by co-localization of both proteins in the cytoplasm of SARS-CoV infected cells.
DOI: 10.1016/j.virusres.2012.07.012
PubMed: 22820404
Affiliations:
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L Pez</name></author><author><name sortKey="Perez Bre A, Pilar" sort="Perez Bre A, Pilar" uniqKey="Perez Bre A P" first="Pilar" last="Pérez-Bre A">Pilar Pérez-Bre A</name></author><author><name sortKey="Falc N, Ana" sort="Falc N, Ana" uniqKey="Falc N A" first="Ana" last="Falc N">Ana Falc N</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22820404</idno><idno type="pmid">22820404</idno><idno type="doi">10.1016/j.virusres.2012.07.012</idno><idno type="wicri:Area/PubMed/Corpus">001333</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001333</idno><idno type="wicri:Area/PubMed/Curation">001333</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001333</idno><idno type="wicri:Area/PubMed/Checkpoint">001285</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001285</idno><idno type="wicri:Area/Ncbi/Merge">002536</idno><idno type="wicri:Area/Ncbi/Curation">002536</idno><idno type="wicri:Area/Ncbi/Checkpoint">002536</idno><idno type="wicri:Area/Main/Merge">001C67</idno><idno type="wicri:Area/Main/Curation">001C55</idno><idno type="wicri:Area/Main/Exploration">001C55</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Severe acute respiratory syndrome coronavirus accessory proteins 6 and 9b interact in vivo.</title><author><name sortKey="Calvo, Enrique" sort="Calvo, Enrique" uniqKey="Calvo E" first="Enrique" last="Calvo">Enrique Calvo</name><affiliation wicri:level="3"><nlm:affiliation>Unidad de Proteómica, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Unidad de Proteómica, Centro Nacional de Investigaciones Cardiovasculares, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Dediego, Marta L" sort="Dediego, Marta L" uniqKey="Dediego M" first="Marta L" last="Dediego">Marta L. Dediego</name></author><author><name sortKey="Garcia, Pilar" sort="Garcia, Pilar" uniqKey="Garcia P" first="Pilar" last="García">Pilar García</name></author><author><name sortKey="L Pez, Juan A" sort="L Pez, Juan A" uniqKey="L Pez J" first="Juan A" last="L Pez">Juan A. L Pez</name></author><author><name sortKey="Perez Bre A, Pilar" sort="Perez Bre A, Pilar" uniqKey="Perez Bre A P" first="Pilar" last="Pérez-Bre A">Pilar Pérez-Bre A</name></author><author><name sortKey="Falc N, Ana" sort="Falc N, Ana" uniqKey="Falc N A" first="Ana" last="Falc N">Ana Falc N</name></author></analytic><series><title level="j">Virus research</title><idno type="eISSN">1872-7492</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Chlorocebus aethiops</term><term>Immunoprecipitation</term><term>Mass Spectrometry</term><term>Molecular Sequence Data</term><term>Protein Interaction Mapping</term><term>SARS Virus (physiology)</term><term>Vero Cells</term><term>Viral Regulatory and Accessory Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cartographie d'interactions entre protéines</term><term>Cellules Vero</term><term>Données de séquences moléculaires</term><term>Immunoprécipitation</term><term>Protéines virales régulatrices ou accessoires (métabolisme)</term><term>Spectrométrie de masse</term><term>Séquence d'acides aminés</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Viral Regulatory and Accessory Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines virales régulatrices ou accessoires</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Chlorocebus aethiops</term><term>Immunoprecipitation</term><term>Mass Spectrometry</term><term>Molecular Sequence Data</term><term>Protein Interaction Mapping</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cartographie d'interactions entre protéines</term><term>Cellules Vero</term><term>Données de séquences moléculaires</term><term>Immunoprécipitation</term><term>Spectrométrie de masse</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The 3'proximal one-third of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes the structural proteins and eight accessory proteins, including 3a, 3b, 6, 7a, 7b, 8a, 8b and 9b, varying in length from 39 to 274aa which do not share significant homology with viral proteins of known coronaviruses. The SARS-CoV protein 6 is 63 amino acids in length and has been previously involved in virus pathogenicity and replication. To further analyze this functions, the interaction of SARS-CoV protein 6 with other viral and/or cellular factors has been analyzed during SARS-CoV infective cycle. Protein 6 immunoprecipitation from extracts of SARS-CoV infected cells and mass spectrometry analysis revealed an interaction of viral proteins 6 and 9b in biologically relevant conditions. This interaction has been reinforced by co-localization of both proteins in the cytoplasm of SARS-CoV infected cells.</div></front></TEI><affiliations><list><country><li>Espagne</li></country><region><li>Communauté de Madrid</li></region><settlement><li>Madrid</li></settlement></list><tree><noCountry><name sortKey="Dediego, Marta L" sort="Dediego, Marta L" uniqKey="Dediego M" first="Marta L" last="Dediego">Marta L. Dediego</name><name sortKey="Falc N, Ana" sort="Falc N, Ana" uniqKey="Falc N A" first="Ana" last="Falc N">Ana Falc N</name><name sortKey="Garcia, Pilar" sort="Garcia, Pilar" uniqKey="Garcia P" first="Pilar" last="García">Pilar García</name><name sortKey="L Pez, Juan A" sort="L Pez, Juan A" uniqKey="L Pez J" first="Juan A" last="L Pez">Juan A. L Pez</name><name sortKey="Perez Bre A, Pilar" sort="Perez Bre A, Pilar" uniqKey="Perez Bre A P" first="Pilar" last="Pérez-Bre A">Pilar Pérez-Bre A</name></noCountry><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Calvo, Enrique" sort="Calvo, Enrique" uniqKey="Calvo E" first="Enrique" last="Calvo">Enrique Calvo</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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